A 20 μl reaction in 1xCas12a Nuclease Reaction Buffer containing 60 ng linearized plasmid, 10 ng crRNA, and 100 ng GenCRISPR™ Cas12a (Cpf1) Nuclease for 30 mins at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
GenCRISPR™ Cas12a (Cpf1) Nuclease
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Z03502 |
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¥915 |
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联系黄金城集团 |