A 20 μl reaction in 1×Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-2NLS for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

GenCRISPR™ Ultra eSpCas9-2NLS-Research

GenCRISPR™ Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.

产品编号	Z03622
规格	0.5 mg 1 mg 5 mg 大单询价
数量
价格	￥9660


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Description

The GenCRISPR™ Ultra eSpCas9 product line provides customers with a selection of research use, basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and transcription/translational challenges.

GenCRISPR™ Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

产品概述

Key Features

High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Tag-free: No extra tag amino acid
Animal-free: Plant-based culture media, Enzyme-free chromatographic purification.
DNA-free: No external DNA added to the system.

应用指导

gRNA-dependent double-stranded DNA cleavage

产品属性

Expression System	Recombinant Cas9 with double-ended NLS expressed by E.coli
Species	S. pyogenes
Tag	Tag-free
Molecular Weight	~160 kDa
Concentration	10 mg/mL
Active temperature	This eSpCas9 is active at 37°C.
Formulation	Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol pH 8.0 at 25°C.
Storage & Stability	This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles.

质控参数

Appearance	Clear, colorless liquid
Purity	≥ 95% as analyzed by SDS-PAGE ≥ 90% as analyzed by SEC-HPLC
Concentration by A280	10 mg/ml±1 mg/ml
Bioactivity (in vitro)	≥ 90%
Residual DNase	Non-specific DNase activity
Residual RNase	Non-specific RNase activity
Endotoxin Level	≤ 10 EU/mg as analyzed by gel clotting method