Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry where false results could be generated in immunological assays from contaminated Protein A.
GenScript Protein A ELISA Kit is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe ^TM in the presence or absence of antibodies. The kit offers pharmaceutical users a sensitive assay to measure mini amount of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of their resins under specific conditions.

Key Features:

• Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe ^TM in antibody preps
• Broad-range recongnition of native Protein A, recombinant Protein A variants and MabSelect SuRe ^TM
• Low level deviation - Intra-assay <5%, Inter-assay <10%
• High accuracy/recovery within 85-115%
• Ready-to-use ELISA plate with fast & simple samples preparation
• Time-saving protocol - complete assay in less than 2 hours
• Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies
• Wide applications for the quantification of Protein A contaminant and leaching

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