eZwest^TM Lite is a fully automated blotting device that frees researchers from the manual labor of traditional western protocol. It can automatically perform blocking, primary antibody and secondary antibody incubations and washing. When desired, the primary antibody can be recovered for re-use. The processed membranes are ready for detection. The eZwest^TM Lite system is compatible with the traditional reagents and protocols used for conventional western blotting. No proprietary reagents are required. Fully customizable steps and solutions make eZwest^TM Lite a powerful tool fitting specific experiment needs for both routine and novel western blotting conditions.