| Linearity Range |
MabSelect PrismA (Cytiva): 0.0625 to 4 ng/mL
AT Protein A (Bestchrom): 0.0625 to 4 ng/mL
MabSelect SuRe (Cytiva): 0.1 to 6.4 ng/mL
UniMab 50HC Protein A (NanoMicro): 0.2 to 12.8 ng/mL
MabPurix Protein A (Srpax): 0.05 to 3.2 ng/mL
rSPA (Repligen) : 0.1 to 6.4 ng/mL |
| Sensitivity |
LLoQ: 0.0625 ng/mL (For MabSelect PrismA)
ULoQ: 4 ng/mL (MabSelect PrismA) |
| Precision |
Intra-assay: CV ≤ 10% (For MabSelect PrismA)
Inter-assay: CV ≤ 15% (For MabSelect PrismA) |
| Accuracy |
80-120% |
| Standard |
1 µg/mL of MabSelect PrismA ligand |
| Assay Time |
1 h 40 min |
| Storage |
The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C. |
| Kit Contents |
| Component |
Quantity/Size |
Part No. |
| Capture Plate |
1 plate |
Y1-80 |
| MabSelect PrismA Stock |
1 vial (0.1 mL) |
Y1-10 |
| Biotin conjugated anti-Protein A Antibody |
1 bottle (12 mL) |
Y1-20 |
| Streptavidin HRP |
1 bottle (12 mL) |
Y1-30 |
| Sample Dilution Buffer |
1 bottle (60 mL) |
Y1-60 |
| Denaturing Solution |
1 bottle (10 mL) |
Y1-91 |
| Detection Buffer |
1 bottle (12 mL) |
Y1-90 |
| 20×Wash Solution |
1 bottle (60 mL) |
B-A1-71 |
| TMB Solution |
1 bottle (12 mL) |
B-A1-40 |
| Stop Solution |
1 bottle (6 mL) |
B-A1-50 |
| Plate Sealer |
2 pieces |
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|
MabSelect PrismA standard curve
Various Protein A ligand standard curves
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
