| Product Description |
Protein
A affinity chromatography is widely used for antibody purification. The leakage
of Protein A ligands has become a significant problem in the pharmaceutical
industry, even when Protein A ligands are covalently bound to chromatography
support. It also probably causes false results in immunological assays if an antibody
is contaminated with Protein A. Therefore, the assessment
of residual Protein A is a critical quality control step in the production
process of antibodies.
Leached
Protein A tends to interact with residual immunoglobulins in the sample through
its Fc binding domain. This interaction hinders the formation of complexes
between the anti-Protein A antibodies and Protein A, thereby interfering with
the accurate quantification of Protein A in immunoassays and leading to a
significant underestimation of Protein A impurities. It is noteworthy that the
extent of this interference varies considerably among different antibodies,
with a particularly common occurrence in many human IgGs.
GenScript Protein A ELISA Kit, 2G (Cat. No. L01043) is designed to
accurately quantify most forms of Protein A available on the market, including native
Protein A, recombinant Protein A variants and Alkali-tolerant recombinant
protein A variants. This kit provides a unique ‘Denaturing Solution’ that
effectively disrupts the binding between Protein A and IgG, thereby minimizing
its interference with quantification. In comparison to traditional boiling
methods for Protein A dissociation, this kit offers a more accurate, simple-to-use,
precise and highly sensitive method for quantifying Protein A, with a detection
limit as low as 62.5 pg/mL, in mouse, rat, rabbit, or human antibody samples.
This
kit can be used to measure trace amounts of Protein A in therapeutic antibody
products in the pharmaceutical industry. Antibody suppliers can use the kit to
evaluate Protein A containment level of the products. It also makes it easier
for the manufacturers of Protein A affinity chromatography media in the process
of monitoring the leaching characteristics of the media under specific
conditions. |
| LLOQ |
0.0625 ng/mL
|
| ULOQ |
4 ng/mL
|
| Kit Contents |
| Component |
Quantity/Size |
Part No. |
| Capture Plate |
1 plate |
Y1-80 |
| MabSelect PrismA Stock |
1 vial (0.1 mL) |
Y1-10 |
| Biotin conjugated anti-Protein A Antibody |
1 bottle (12 mL) |
Y1-20 |
| Streptavidin HRP |
1 bottle (12 mL) |
Y1-30 |
| Sample Dilution Buffer |
1 bottle (60 mL) |
Y1-60 |
| Denaturing Solution |
1 bottle (10 mL) |
Y1-91 |
| Detection Buffer |
1 bottle (12 mL) |
Y1-90 |
| 20×Wash Solution |
1 bottle (60 mL) |
Y1-70 |
| TMB Solution |
1 bottle (12 mL) |
A1-40 |
| Stop Solution |
1 bottle (6 mL) |
A1-50 |
| Plate Sealer |
2 pieces |
/ |
|
| Storage |
The
unopened kit is stable for at least 12 months from the date of manufacture at
2°C to 8°C, and the opened kit is stable for up to 1 month from the date of
opening at 2°C to 8°C. |
MabSelect PrismA standard curve
Various Protein A ligand standard curves
For research use
only. Not intended for human and animal therapeutic or diagnostic use.
