| Product Description |
Staphylococcal
Protein A binds strongly and specifically to the Fc region of immunoglobulin
molecules, especially IgG of many species such as Human, Rabbit, Pig, etc. The
product contains five high affinity binding sites that are capable of
interacting with the Fc region of IgG. HRP-Protein A is produced by covalently linking
HRP to Protein A, which can be used in immuno-detection such as immunoblotting
(western or dot blot), ELISA, and immunohistochemistry. |
| Reconstitution |
Reconstitute
the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final
HRP-Protein A concentration of 1 mg/ml. |
| Storage |
The product
remains stable in lyophilized form for at least 1 year if stored at 2-8°C,
-20°C, or lower from the date of receipt. For longer storage, -20°C or below is
recommended.
The
reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to 1 year
at -20°C or below. Avoid repeated freezing and thawing cycles. |
|
This product is
suitable for immunoblotting (Western or Dot blot), ELISA, immunoperoxidase
electron microscopy, and immunohistochemistry as well as other
peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each
specific application. We have not yet determined the suitability of this
antibody for applications other than those listed below. The ideal working
concentration must take into account such factors as secondary antibody
affinity, antigen concentration, sensitivity of the detection method,
temperature, and the length of the incubations. The suitability of this antibody
for applications other than those listed below has not been determined. The
following concentration ranges are recommended starting points for this
product. |
| Application |
Recommended Usage |
| Dot blot |
1:5,000-1:20,000 |
| Western blot |
1:5,000-1:20,000 |
| ELISA |
1:50,000-1:250,000 |
| Immunohistology |
1:500-1:2,500 |
|
For research use only.
Not intended for human and animal therapeutic or diagnostic use.
