HRP-Protein L

Protein L is a cell surface protein expressed by Peptostreptoccocus magnus, that binds to the variable light chains (kappa chains) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. HRP-Protein L is produced by covalently linking HRP to protein L. The complex can be used in immuno-detection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.

产品编号	M00098
规格	1 mg 大单询价
数量
价格	￥950


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产品属性

Reconstitution	Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.
Storage	The product remains stable in lyophilized form for at least 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to 1 year at -20°C or below. Avoid repeated freezing and thawing cycles.

应用指导

The ideal working concentrations for each specific application should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time. The following dilution ranges are recommended starting points:

Application	Recommended Usage
Dot blot	1:2,000-1:20,000
Western blot	1:2,000-1:20,000
ELISA	1:20,000-1:200,000
Other applications	user-optimized

技术背景

Target Background

Protein L is a cell surface protein expressed by Peptostreptoccocus magnus, that binds to the variable light chains (kappa chains) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. HRP-Protein L is produced by covalently linking HRP to protein L. The complex can be used in immuno-detection such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.