Figure 1. CP-55940-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CB1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist CP-55940. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CP-55940 (Mean ± SEM, n = 3). The EC₅₀ of CP-55940 on this cell was 16.10 μM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CP-55940 in CHO-K1/Gα15/CB1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/Gα15/CB1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of CP-55940 on CHO-K1/Gα15/CB1 cells was 84.45 nM.

Figure 3. FACS analysis of cell surface expression of CB1 on CHO-K1/Gα15/CB1 cells. The CHO-K1/Gα15/CB1 cells were probed using anti-CB1 Nimacimab and Alexa Fluor 647-conjugated goat anti-human IgG (H+L) secondary antibody (Cat. No. A21445, Thermo Fisher).

CHO-K1/CB1/Gα15 Stable Cell Line

The cannabinoid receptor CNR1 is Gs-coupled GPCRs expressed in primarily CNS and some peripheral neurones; particularly prevalent in basal ganglia, hippocampus, cerebellum, cerebral cortex and also presented in some non-neuronal cells and tissues, for example leukocytes and testis. A shorter human splice variant (411aa) has been identified in the brain and other tissues by reverse-transcriptase PCR but mRNA levels were less than 10-fold the levels of the longer isoform. The pharmacological characteristics of the isoforms are similar.

产品编号	M00299
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant CHO-K1 cells stably overexpress human cannabinoid receptor 1 (CB1) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1. CP-55940-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CB1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist CP-55940. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CP-55940 (Mean ± SEM, n = 3). The EC₅₀ of CP-55940 on this cell was 16.10 μM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with CP-55940 in CHO-K1/Gα15/CB1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/Gα15/CB1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of CP-55940 on CHO-K1/Gα15/CB1 cells was 84.45 nM.

Figure 3. FACS analysis of cell surface expression of CB1 on CHO-K1/Gα15/CB1 cells. The CHO-K1/Gα15/CB1 cells were probed using anti-CB1 Nimacimab and Alexa Fluor 647-conjugated goat anti-human IgG (H+L) secondary antibody (Cat. No. A21445, Thermo Fisher).

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/CB1/Gα15 Stable Cell Line

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