Description
Broad Multi Color Pre-Stained Protein Standard
is a special-designed protein standard. The standard is a mixture of ten highly
purified pre-stained proteins ranging from 10 kDa to 270 kDa, covalently
coupled with three different chromophores. The 30 kDa and 270 kDa bands are orange,
50 kDa band is green, and other bands are blue. Broad Multi Color Pre-Stained Protein Standard
is designed for observing protein separation during SDS-PAGE, verifying western
transfer efficiency on membranes, and approximating the size of proteins. Due
to the influence of covalently coupled chromophores on the electrophoretic
mobility of proteins, this product is only suitable for roughly
estimating the molecular weight of target protein samples. |
Storage Buffer |
62.5 mM Tris-H3PO4 (pH 7.5), 1 mM EDTA, 2% SDS, 10
mM DTT, 1 mM NaN3, 33% glycerol. |
Storage |
Stable at -20°C for up to 24 months or 4°C for up to 3 months. Aliquots
are recommended before use to avoid repeated freeze-thaw cycles. |
Procedures |
1. Thaw this product
at room temperature. Do NOT boil protein marker. 2. Mix gently and
thoroughly to ensure that the solution is homogeneous. 3. Bands intensity
may vary according to gel type, thickness, and staining condition. Please
adjust loading volume from 2.5 µl to 10 µl. |
Recommendations |
1. Completely thaw
and mix this product at room temperature before using. 2. This product is
only suitable for roughly estimating the molecular weight of protein samples.
|
1. Monitoring
protein migration during SDS-PAGE. 2. Estimating
protein sizes on SDS-PAGE gels. 3. Verifying
transfer efficiency on membranes for Western Blot. 4. Locating a
protein of interest for excision from an unstained preparative gel. |
For laboratory research use only. Direct human
use, including taking orally and injection and clinical use are forbidden.