黄金城集团

概览

主要用于全蛋白扫描,为筛选线性或连续性表位提供理想工具。根据目的蛋白或长肽设计关键短肽序列,可用来鉴定蛋白活性、免疫特异性以及抗体结合性。重叠肽库的设计主要由肽链长度和步移(offset number)两个参数决定,从目的蛋白N端逐步向C端截取设计,每次转移一个或几个氨基酸,序列之间有部分重合,最终形成重叠肽库。

选择合适的步移和肽段长度可以降低实验成本,同时使数据价值最大化。步移通常设计为肽段长度的1/3。长肽段会包含更多的表位,但是一般这类肽段很难合成,并且库中包含的肽段变少。短肽段更容易合成,也更便宜,但每肽段的表位较少。短步移数和短肽组合可以形成最多数量的多肽,而长步移数与长肽组合产生的多肽数量最少。

应用领域

鉴定抗原肽序列

筛选酶底物

鉴定T细胞表位

Overlapping Peptide
overlapping library
 
Alanine Scanning
alanine scanning library
     
Truncation Library
truncation library
 
Positional Scanning
positional scanning library
     
Random Library
random library
 
Scrambled Library
scrambled library
     
T Cell Truncated Library
T tell truncated tibrary
   

参考文献

    Sospedra M, Pinilla C, and Martin R. Use of combinatorial peptide libraries for T-cell epitope mapping. Methods. Mar 2003; 29(3): 236-47

    Gershoni JM, Roitburd-Berman A, Siman-Tov DD, Tarnovitski Freund N, and Weiss Y. Epitope mapping: the first step in developing epitope-based vaccines. BioDrugs. 2007; 21(3): 145-56

    Hemmer B, Pinilla C, Appel J, Pascal J, Houghten R, and Martin R. The use of soluble synthetic peptide combinatorial libraries to determine antigen recognition of T cells. J. Pept. Res. Nov 1998; 52(5): 338-45

    Sung MH, Zhao Y, Martin R, and Simon R. T-cell epitope prediction with combinatorial peptide libraries. J. Comput. Biol. 2002; 9(3): 527-39

    Paulmurugan R, and Gambhir SS. Combinatorial library screening for developing an improved split-firefly luciferase fragment-assisted complementation system for studying protein-protein interactions. Anal. Chem. Mar 2007; 15; 79(6): 2346-53

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