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HEK293/STAT6-RE-Luc Stable Cell Line

Recombinant HEK293 cells stably express human interleukin 4 receptor (IL - 4R) on the surface. The luciferase reporter gene was inserted into the genome of the cell under the control of STAT6 - RE. The cytokine samples (IL - 4 and IL - 13) can activate the receptor and transmit this signal to the downstream signaling pathways. The surface expression of IL - 4R is validated by luminescence analysis. This stable cell line product is designed for In Vitro cell - based functional assays to determine the bioactivity of IL - 4 and IL - 13.
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M00949

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Product Description Recombinant HEK293 cells stably express human interleukin 4 receptor (IL - 4R) on the surface. The luciferase reporter gene was inserted into the genome of the cell under the control of STAT6 - RE. The cytokine samples (IL - 4 and IL - 13) can activate the receptor and transmit this signal to the downstream signaling pathways. The surface expression of IL - 4R is validated by luminescence analysis. This stable cell line product is designed for In Vitro cell - based functional assays to determine the bioactivity of IL - 4 and IL - 13.
Culture Properties Adherent
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
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Culture Medium DMEM, 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-02, Life Technologies), 300 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 200 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies), 100 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium DMEM, 10% FBS
Freeze Medium-DATA 42% DMEM (Cat. No. 10566, Life Technologies), 50% FBS (Cat. No. 10099-141, Life Technologies), 8% DMSO (Cat. No. D2650, Sigma)
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HEK293/STAT6-RE-Luc Stable Cell Line

Figure 1. IL-4-induced luciferase expression in HEK293/STAT6-RE-Luc cells. After stimulation by agonist IL-4 (Cat. No. Z02925, Genscript) for 6 hours, the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of IL-4 (Mean ± SEM, n = 3). The EC50 of IL-4 on this cell was 28.72 ng/ml.

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HEK293/STAT6-RE-Luc Stable Cell Line

Figure 2. IL-13-induced luciferase expression in HEK293/STAT6-RE-Luc cells. After stimulation by agonist IL-13 (Cat. No. Z03020, Genscript) for 6 hours, the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of IL-13 (Mean ± SEM, n = 3). The EC50 of IL-13 on this cell was 29.27 ng/ml.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RLU and starts at Bottom and goes to Top along a sigmoid curve. »

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 For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.