ED₅₀<0.4 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 10 µg/mL heparin, corresponding to a specific activity of > 2.5× 10⁶ units/mg.

FGF-acidic, Mouse

Fibroblast Growth Factor- acidic (FGF-acidic) is a mitogen targeting at the endothelial cells, and belongs to the heparin binding FGF family, which contains 22 members. FGF-acidic binds to the receptor family FGFR1-4 in vivo with the assistance of heparin. However, along with FGF -basic, FGF-acidic lacks the signal peptide segment, and thus is not secreted via endoplasmic reticulum (ER) and Golgi bodies. Studies have shown that FGF-acidic is highly regulated, and it is a direct angiogenesis factor. If unregulated, angiogenesis could contribute to several diseases including arthritis, diabetes, ocular neovascularization, and especially tumors. Therefore, FGF-acidic is treated as a potential oncogene, and its overexpression is correlated tightly with several cancers.

产品编号	Z03049
规格	10 μg 50 μg 1 mg 大单询价
数量
价格	￥300


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产品简介

Species

Mouse

Protein Construction

FGF-acidic (Phe16-Asp155)
Accession # P61148

Purity

> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.4 ng/ml, measured by a cell proliferation assay using 3T3 cells in the presence of 10.0 µg/ml heparin, corresponding to a specific activity of > 2.5 × 10⁶ units/mg.

Expression System

E. coli

Apparent Molecular Weight

~15.8 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例

ED₅₀<0.4 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 10 µg/mL heparin, corresponding to a specific activity of > 2.5× 10⁶ units/mg.

技术背景

Target Background

Synonyms

Fibroblast Growth Factor-acidic; HBGF-1; ECGF-beta; FIBP; FGFIBP; FIBP-1; ECGF; ECGFA; GLIO703; FGF1; FGF-a

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.