| Species |
Mouse |
| Protein Construction |
FGF-10 (Ser62-Thr209)
Accession # O35565 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 10.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0 × 105 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~17 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
ED₅₀< 10 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 1.0× 10⁵ units/mg.
| Target Background |
Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF, and both KGF and FGF-10 activate the receptor FGFR2-IIIb. However, unlike KGF, which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during multi-organ development via mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development and is regulated by Shh during early development. |
| Synonyms |
Fibroblast Growth Factor-10; FGFA; Keratinocyte growth factor-2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
