| Species |
Rat |
| Protein Construction |
Expressed with an N-terminal Met.
PDGF-BB (Ser74-Thr182)
Accession # Q05028 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 2.0 ng/ml, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 5.0 × 105 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~24.6 kDa, on SDS-PAGE under non-reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against 20 mM acetic acid. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in 20 mM acetic acid up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Lane 1: 2 μg of PDGF-BB, Rat, non-reducing (NR)
Lane 2: 2 μg of PDGF-BB, Rat, reducing (R)
> 95% as analyzed by SDS-PAGE
| Target Background |
Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets. |
| Synonyms |
PDGFBB; IBGC5; PDGF-2; PDGF2; SIS; SSV; c-sis; platelet derived growth factor subunit B |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
