| Species |
Rat |
| Protein Construction |
IL-12 p35 (Arg23-Ser215)
Accession # Q9R103 |
Poly-His |
IL-12 p40 (Met23-Ser335)
Accession # E9PU71 |
|
| N-term |
C-term |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 0.1 ng/ml, measured in a cell proliferation assay using 2D6 cells. |
| Expression System |
CHO |
| Apparent Molecular Weight |
26~28 kDa (p35), 42~45 kDa (p40), on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals through the IL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2. |
| Synonyms |
IL12; NKSF; TCMF; CLMF; TSF; CTL maturation factor; Cytotoxic lymphocyte maturation factor |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
