| Species |
Human |
| Protein Construction |
Expressed with an N-terminal Met.
| Poly-His |
sIL-6Rα (Leu20-Asp358)
Accession # P08887-1 |
| N-term |
C-term |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 50.0 ng/ml, measured by the cytotoxicity assay using M1 cells in presence of 10.0 ng/ml of human IL-6, corresponding to a specific activity of > 2.0 × 104 units/mg. |
| Expression System |
Sf9 insect cells |
| Apparent Molecular Weight |
~50 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin-6 Receptor (IL-6R) is a single trans-membrane protein that is the receptor for Interleukin-6 (IL-6). IL-6R forms a hexameric complex upon binding 2 molecules of IL-6 and two molecules of glycoprotein 130 (gp130) which activates intracellular JAK/STAT pathways. Although the normal form of IL-6R is the membrane-bound 80 kDa subunit, a soluble form of IL-6R (sIL-6R) can be generated physiologically by limited proteolysis or alternative splicing. sIL-6R binds to both IL-6 and gp130 generating intracellular signaling. In the immune system, sIL-6R is produced by both naïve and memory CD4 T-cells and strongly augments IL-6 ligand’s induction of Th-17 cells. |
| Synonyms |
Interleukin-6 receptor subunit alpha; IL-6R subunit alpha; IL-6RA; IL-6R 1; Membrane glycoprotein 80; gp80; CD_antigen: CD126; sIL6RA |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
