| Species |
Mouse |
| Protein Construction |
MCP-1 (Gln24-Arg96)
Accession # Q5SVU3 |
|
| Purity |
> 98% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
The EC50 value of mouse MCP-1/CCL2 on Ca2+ mobilization assay in CHO-K1/Ga15/mCCR2 cells (human Ga15 and mouse CCR2 stably expressed in CHO-K1 cells) is less than 0.3 μg/ml. |
| Expression System |
HEK 293 |
| Apparent Molecular Weight |
~8 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemotactic protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. CCL2 is implicated in the pathogeneses of several types of disease characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. CCL2 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. CCL2 is primarily secreted by monocytes, macrophages and dendritic cells. CCL2 can signal through the CCR2 receptor. |
| Synonyms |
MCAF; JE; Monocyte Chemotactic Protein-1; CCL2; MCP1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
