| Species |
Human |
| Protein Construction |
| Poly-His |
IL-2 Rα (Glu22-Cys213)
Accession # P01589 |
| N-term |
C-term |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1.0 ng/ml of recombinant IL-2, ED50 for this effect is < 1.5 µg/ml. |
| Expression System |
HEK 293 |
| Apparent Molecular Weight |
~42 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to the cytokine IL-2. The IL-2R is made up of 3 subunits - alpha (α), beta (β) and gamma (γ). The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. The β and γ chains of the IL-2R are members of the type I cytokine receptor family. IL-2R has a high binding affinity to IL-2 and is expressed by antigen-activated T lymphocytes (T cells). IL-2 Rα is also known as CD25, p55, and Tac (activated T cell) antigen. |
| Synonyms |
IL2RA; soluble IL-2 receptor; TAC-antigen; CD25 antigen; Tac antigen; IL-2Rα |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
