![GenCrispr NLS-Cas9-EGFP Nuclease GenCrispr NLS-Cas9-EGFP Nuclease](/product/documents/down?doc_name=activity.png&file=scm_files/productFile_notes/2016/08/16/53a4fb230184099d652ac4706503965f.png)
In vitro DNA cleavage assay with GenCrispr NLS-Cas9-EGFP nuclease
Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is EcoR V-linearized pUC57 plasmid DNA. Lane 1, DNA + gRNA; lane 2, marker; lane 3 and 4, DNA + gRNA + NLS-Cas9-EGFP 100 ng (lane 3) or 50 ng (lane 4).
![GenCrispr NLS-Cas9-EGFP Nuclease GenCrispr NLS-Cas9-EGFP Nuclease](/product/documents/down?doc_name=fluorescence.jpg&file=scm_files/productFile_notes/2016/08/16/67a5a785759a9df13f0a3ec005c0f138.jpg)
Left: Cell transfection assay. 12 h after electroporation, cells were observed under bright or fluorescence microscope.
Right: in vivo gene editing efficiency assay by T7E1. Lane 1, marker; lane 2, negative control; lane 3, gRNA + NLS-Cas9-EGFP; lane 4, gRNA + NLS-Cas9.
![GenCrispr NLS-Cas9-EGFP Nuclease GenCrispr NLS-Cas9-EGFP Nuclease](/product/documents/down?doc_name=Z03393_digestion Efficiency.png&file=scm_files/productFile_notes/2024/01/17/8d2bf56d-b489-478f-85d4-777db617c1af.png)
A 20 μl reaction in 1 × Cas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and NLS-Cas9-EGFP for 1 hour at 37 °C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
GenCrispr NLS-Cas9-EGFP Nuclease
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Z03393 |
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¥915 |
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联系黄金城集团 |