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A Conserved Patch near the C Terminus of Histone H4 Is Required for Genome Stability in Budding Yeast.

Mol Cell Biol.. 2011-06;  31(11):2311 - 2325
Yao Yu, Madhusudhan Srinivasan, Shima Nakanishi, Janet Leatherwood, Ali Shilatifard, and Rolf Sternglanz. Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, USA.
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摘要

A screen of Saccharomyces cerevisiae histone alanine substitution mutants revealed that mutations in any of three adjacent residues, L97, Y98, or G99, near the C terminus of H4 led to a unique phenotype. The mutants grew slowly, became polyploid or aneuploid rapidly, and also lost chromosomes at a high rate, most likely because their kinetochores were not assembled properly. There was lower histone occupancy, not only in the centromeric region, but also throughout the genome for the H4 mutants. The mutants displayed genetic interactions with the genes encoding two different histone chaperones, Rtt106 and CAF-I. Affinity purification of Rtt106 and CAF-I from yeast showed that much more H4 and H3 were bound to th... More

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