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Identification of the Atypical MAPK Erk3 as a Novel Substrate for p21-activated Kinase (Pak) Activity.

J Biol Chem.. 2011-04;  286(15):13603 - 13611
Alina De La Mota-Peynado, Jonathan Chernoff, and Alexander Beeser. Division of Biology and Molecular, Cellular, and Developmental Biology Program, Kansas State University, Manhattan, Kansas 66506, USA.
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摘要

The class I p21-activated kinases (Pak1-3) regulate many essential biological processes, including cytoskeletal rearrangement, cell cycle progression, apoptosis, and cellular transformation. Although many Pak substrates, including elements of MAPK signaling cascades, have been identified, it is likely that additional substrates remain to be discovered. Identification of such substrates, and determination of the consequences of their phosphorylation, is essential for a better understanding of class I Pak activity. To identify novel class I Pak substrates, we used recombinant Pak2 to screen high density protein microarrays. This approach identified the atypical MAPK Erk3 as a potential Pak2 substrate. Solution-ba... More

关键词

Enzyme Purification; MAPKs; Protein Kinases; Protein-Protein Interactions; Signal Transduction; Atypical MAPK; p21-activated Kinase