Products/Services Used | Details | Operation |
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Recombinant Proteins> | Antiserum was purified by the His6-Nrv3 fusion protein. All three antibodies were generated by GenScript (Nanjing, China). Other antibodies used in this study were: HA-tag (3724; Cell Signaling Technology, Danvers, MA, USA); PDF (C7; Developmental Studies Hybridoma Bank, Iowa City, IA, USA); Tubulin (T6199; Sigma, St. Louis, MO, USA); Actin (R15006MC4; Yurogen, Worcester, MA, USA); Myc-tag (Sigma, M4439); Ubi (ab7254; Abcam, Cambridge, UK); and His-tag (A00186; GenScript, Piscataway, NJ, USA). | Get A Quote |
The timing of sleep is tightly governed by the circadian clock, which contains a negative transcriptional feedback loop and synchronizes the physiology and behavior of most animals to daily environmental oscillations. However, how the circadian clock determines the timing of sleep is largely unclear. In vertebrates and invertebrates, the status of sleep and wakefulness is modulated by the electrical activity of pacemaker neurons that are circadian regulated and suppressed by inhibitory GABAergic inputs. Here, we showed that Drosophila GABA receptors undergo rhythmic degradation in arousal-promoting large ventral lateral neurons (lLNvs) and their expression level in lLNvs displays a daily oscillation. We... More
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