黄金城集团

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Chromogranin B regulates early-stage insulin granule trafficking from the Golgi in pancreatic islet β-cells.

J Cell Sci. 2019-07; 
BearrowsShelby C,BauchleCasey J,BeckerMcKenzie,HaldemanJonathan M,SwaminathanSvetha,StephensSamu
Products/Services Used Details Operation
Monoclonal Antibody Services … 5000), PC2 (rabbit, Thermo Scientific PA1-058, 1:1000), proinsulin (mouse monoclonal GS-9A8, Developmental Studies Hybridoma Bank, University of Iowa, 1:500), SNAP-tag (rabbit, GenScript A00684-40), and VGF (rabbit … Get A Quote

摘要

Chromogranin B (CgB, also known as CHGB) is abundantly expressed in dense core secretory granules of multiple endocrine tissues and has been suggested to regulate granule biogenesis in some cell types, including the pancreatic islet β-cell, though the mechanisms are poorly understood. Here, we demonstrate a critical role for CgB in regulating secretory granule trafficking in the β-cell. Loss of CgB impairs glucose-stimulated insulin secretion, impedes proinsulin processing to yield increased proinsulin content, and alters the density of insulin-containing granules. Using an fluorescent pulse-chase strategy to track nascent proinsulin, we show that loss of CgB impairs Golgi budding of proinsulin... More

关键词

Chromogranin B,Granule trafficking,Insulin secretion,Secretory granule biogenesis,β-cell func