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SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis.

Cells. 2019; 
Wang P,,,, Xia J,, Zhang L,, Zhao S,, Li S,, Wang H,, Cheng S, Li H,, Yin W,, Pei D,,, Shu X,,,,.
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Catalog Antibody … After centrifugation at 3600 rpm for 4 min, supernatants (450 µL) were collected and incubated with the PCM1 antibody (5 µg) overnight at 4 °C, and then incubated with the Protein A Resin (50 µL, L00210, Genscript, Piscataway, NJ, USA) for 4 h at 4 °C. For pull-down of Flag … Get A Quote

摘要

Centriolar satellites are non-membrane cytoplasmic granules that deliver proteins to centrosome during centrosome biogenesis and ciliogenesis. Centriolar satellites are highly dynamic during cell cycle or ciliogenesis and how they are regulated remains largely unknown. We report here that sorting nexin 17 (SNX17) regulates the homeostasis of a subset of centriolar satellite proteins including PCM1, CEP131, and OFD1 during serum-starvation-induced ciliogenesis. Mechanistically, SNX17 recruits the deubiquitinating enzyme USP9X to antagonize the mindbomb 1 (MIB1)-induced ubiquitination and degradation of PCM1. SNX17 deficiency leads to enhanced degradation of USP9X as well as PCM1 and disrupts ciliogenesis upon se... More

关键词

MIB1; PCM1; SNX17; USP9X; centriolar satellite; cilia