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Delineation of Domains Within the Cannabinoid CB1 and Dopamine D2 Receptors That Mediate the Formation of the Heterodimer Complex.

J Mol Neurosci.. 2013-11; 
SS Khan, FJS Lee. Faculty of Health Sciences, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada.
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摘要

Both the cannabinoid CB1 receptor (CB1) and dopamine D2 receptor (D2R) are G protein-coupled receptors that are linked to inhibitory Gαi/o protein, whereby activation of the receptor leads to the inhibition of cAMP production. Moreover, previous findings have shown evidence of cross-talk between the dopamine and endocannabinoid systems. In this report, we confirm the interaction of CB1 and D2R with co-immunoprecipitation experiments using human embryonic kidney 293T (HEK-293T) cells co-expressing both receptors. We also generated GST and His-tagged fusion proteins of the D2R and CB1 and conducted affinity purification assays and in vitro binding experiments to show that the CB1-D2R complex can be formed b... More

关键词

Dopamine; Cannabinoid; G protein-coupled receptor; Protein-protein interaction