| Specificity |
The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A01967-40, MonoRab Anti-Obinutuzumab Antibody (169F10), mAb, Rabbit. |
| Host Species |
Rabbit |
| Immunogen |
Obinutuzumab |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
8G12 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
Standard curve of Obinutuzumab Sandwich ELISA. The Obinutuzumab Sandwich ELISA assay is developed by using MonoRabᵀᴹ Anti-Obinutuzumab Antibody (8G12), mAb, Rabbit (GenScript, A01966-40) and MonoRabᵀᴹ Anti-Obinutuzumab Antibody (169F10) (Genscript, A01967-40) as the capture and detection antibodies, respectively. These two antibodies recognize different epitopes.
In this ELISA assay, MonoRabᵀᴹ Anti-Obinutuzumab Antibody (169F10) (GenScript, A01967-40) was labeled with Biotin. GenScript can provide customized conjugation services for this product per the customer's request.
The sensitivity is less than 100 pg/ml.
ELISA binding of MonoRabᵀᴹ Anti-Obinutuzumab Antibody (8G12) (GenScript, A01966-40) with Obinutuzumab. While the antibody does not recognize the human IgG (data not shown).
Coating antigen: Obinutuzumab, 1 µg/ml.
MonoRabᵀᴹ Anti-Obinutuzumab Antibody (8G12) (GenScript, A01966-40) dilutions start from 2,000 ng/ml.
EC₅₀= 3.230 ng/ml.
| Target Background |
Obinutuzumab (Gazyva) is a humanized monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of chronic lymphocytic leukemia and follicular lymphoma. It binds to a unique conformation epitope on CD20 protein, which partially overlaps with the section recognized by rituximab. When Obinutuzumab binds to CD20 on B cells, it makes these cells to be destroyed by the adaptive immune system. GenScript MonoRab™ Anti-Obinutuzumab Antibody (8G12), mAb, Rabbit is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with Obinutuzumab. |
| Synonyms |
Rabbit monoclonal to Gazyva |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
