Description
Enterokinase (EK) is a serine protease produced
by cells of the duodenum, and is involved in mammalian digestion. It plays the role
of turning trypsinogen to the active form of trypsin, and indirectly activates
the pancreatic digestive enzymes. Enterokinase is a specific protease that
cleaves after a lysine at its cleavage site: Asp-Asp-Asp-Asp-Lys. Enterokinase does
not function if the cleavage site is followed by a proline.
GenScript’s Enterokinase Animal-Free, His,
Bovine is the light chain catalytic subunit of bovine enterokinase consisting
of a single glycosylated polypeptide chain containing Ile801-His1035 (Accession
# P98072). This recombinant protein is expressed in P. pastoris and purified to obtain high yields of the active
enzyme. It is produced under an animal free process, and is suitable for drug and
vaccine development, manufacture and other applications. This product is
designed with a C-terminal 6 × His tag and can be removed after cleavage by Ni2+ affinity
chromatography in resin (Cat. No. L00223) or magnetic
beads (Cat. No. L00295).
|
| Source |
Recombinant Enterokinase expressed in P. pastoris
|
| Species |
Bovine
|
| Tag |
His Tag
|
| Molecular Weight |
Predicted MW: 27 kDa
Apparent MW: ~40 kDa, on SDS-PAGE under reducing
conditions
|
| Purity |
≥ 95% as analyzed by SDS-PAGE
|
| Endotoxin Level |
< 1.0 EU/μg of protein by gel clotting method
|
| Biological Activity |
5 U/μl
Unit definition: One unit is defined as the amount of enzyme sufficient
to cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95%
completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mM
NaCl, 2 mM CaCl2, pH 7.4.
|
| Formulation |
Supplied as solution in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2,
50% glycerol, pH 7.4.
|
| Storage Buffer |
20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH
7.4.
|
| Storage & Stability |
Upon
receiving, the product remains stable for up to 6 months at -20 °C. This
product is stable for up to 1 week at 37 °C. Avoid repeated freeze-thaw cycles
by making single-use aliquots before the solution is stored at -20 °C.
|
For laboratory research or manufacture use only. Direct human
use, including taking orally and injection and clinical use are forbidden.
