Product Description |
Interleukin‐8 (IL‐8), a member of a family of
proinflammatory cytokines, is a neutrophil chemotactic cytokine that can signal through the CXCR1 and CXCR2 receptor.
It is proinflammatory and primarily mediates the activation and migration of
neutrophils from peripheral blood into the sites of inflammation, injury, or infection
in the tissue. IL‐8 is involved in a wide variety
of physiological and
pathological processes, including host defense against bacterial infection,
bronchiolitis, arteriosclerosis, autoimmune disorders of skin, and
angiogenesis‐dependent disorders such as tumor growth, and wound repair. IL‐8
is produced by stimulated monocytes, leukocytic cells, non‐leukocytic somatic
cells and tumor cells. GenScript
Human IL‐8 ELISA Detection Kit can be applied for quantitative detection of
Human IL‐8 concentrations in plasma, blood and cell culture supernatant
samples. The assay utilizes solid sandwich enzyme immunoassay in which two anti
IL‐8 monoclonal antibodies function
as bridge linkers. IL‐8 Capture Plate, which is a 96‐well plate pre‐coated with one anti IL‐8 monoclonal antibody, is used for capture
of IL‐8 in serial IL‐8
standard solutions and sample solutions. IL‐8
Detection Reagent, which is a horseradish peroxidase (HRP) enzyme conjugated to
the other anti IL‐8 monoclonal antibody, is used for enzyme reaction. In the
assay, IL‐8 molecular is fixed between the Capture
Plate and the Detection
Reagent to form a sandwich complex
vial both antibodies. Other unbound moleculars can be removed by several
washings. The IL‐8 Detection Reagent reacts with TMB Solution that provides
absorbance value, which can be obtained by Microplate reader. In optimized test
condition, each absorbance value is indicated to the individual IL‐8 amount in
solution. The IL‐8 standards of known concentration and corresponding
absorbance values are used to form a standard curve. With the standard curve,
IL‐8 amount present in the unknown sample is calculated by transforming its
absorbance value. |
Key Features |
Sensitivity: 2.8 pg/ml Detection Range:
25~1600 pg/ml Test Samples: serum, plasma
and cell culture
supernatant Time Saving Protocol ‐ complete test in 2.5 hours Ready‐to‐use ELISA plate with fast &
simple sample preparation Lot‐to‐lot consistency guaranteed by strict
procedure |
Sensitivity |
The minimum detectable dose (MDD) of IL‐8 was 2.8 pg/ml.
MDD is defined as the concentration
corresponding to a signal three standard deviations above the mean of the zero
standard. |
Kit Contents |
Components |
Quantity |
Part.No |
IL‐8 Capture Plate |
1 plate (8 wells x 12 strips) |
417‐80 |
IL‐8 Detection Reagent |
15 ml |
417‐30 |
IL‐8 Standard |
2 vials |
417‐10 |
Sample Diluent |
30 ml |
417‐60 |
Assay Diluent |
15 ml |
417‐90 |
20× Wash Solution |
40 ml |
B-A1-70 |
TMB Solution |
12 ml |
B-A1-40 |
Stop Solution |
6 ml |
B-A1-50 |
Adhesive Plate Cover |
2 pieces |
N/A |
|
Storage |
The reagents in the kit are stable
for 12 months when stored
at 2‐8°C. It should not be used beyond the expiration date. Do not freeze the
kit. |
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.