Description
Principle
Add the sample containing the HA-tagged protein
to the Anti-HA IP Resin and allow the protein bind to the Resin during a short
time incubation. Then the HA-tagged protein can be eluted off the beads, or
used directly for pull-down of target protein.
Material Supplied
Genscript Anti-HA IP Resin is an agarose beads
coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244).
The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion
proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM
sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).
Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at
-20°C.Do not store the gel at freezing temperatures in the absence of glycerol.
Store the Column: Wash the column three times
with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml
of TBS/A and store at 2-8°C without draining.
Additional Buffers Needed
Equilibration buffer: 50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at
25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.
|
| Product Information |
Characteristics of Anti-HA IP Resin
| Resin Volume | 1 ml settled resin (2 ml 50% slurry) | | Ligand | Anti-HA monoclonal antibody against HA tag | | Number of HA-tagged protein per ligand | 2 | | Binding capacity | Approximately 1 mg HA-tagged protein (~20kDa) per ml settled resin | | Storage solution | 1X PBS containing 50 % glycerol | | Storage and stability | Store at -20 °C for up to 12 months. | | Resin reuse | The resin can be recycled for at least 3 times. If
maintained properly, the resin can be reused 10 times with minimum loss of
binding capability. |
|
Lanes:
M.Protein Marker
1. Whole cell lysate
2. HA tagged fusion protein was precipitated by Anti-HA IP Resin
For
laboratory research use only. Direct human use, including taking orally and
injection and clinical use are forbidden.
