Linearity Range |
31.25 to 2000 pg/mL |
Sensitivity |
LoB: 14.13 pg/mL
LoD: 29.42 pg/mL
LoQ: 39.03 pg/mL |
Precision |
Intra-assay: CV ≤ 10%
Inter-assay: CV ≤ 15% |
Accuracy |
80%-120% |
Standard |
2 µg/mL of recombinant p24 protein |
Suitable Sample Type |
Lentivirus supernatant |
Assay Temperature |
25 °C |
Assay Time |
3 h |
Storage |
The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C. |
Kit Contents |
Component |
Quantity/Size |
Part No. |
Capture Plate |
1 plate |
E1-80 |
Standard Stock |
1 vial (100 μL) |
E1-10 |
Lysis Solution |
1 vial (1.6 mL) |
E1-A0 |
Biotin Anti-p24 Antibody |
1 bottle (12 mL) |
E1-20 |
Streptavidin-HRP |
1 bottle (12 mL) |
E1-30 |
Lentivirus Free p24 Removal Reagent |
1 bottle (2 mL) |
E1-40 |
10× Beads Wash Solution |
1 bottle (40 mL) |
E1-50 |
TMB Solution |
1 bottle (12 mL) |
B-A1-40 |
20× Wash Solution |
1 bottle (40 mL) |
B-A1-70 |
Stop Solution |
1 bottle (6 mL) |
B-A1-50 |
Plate Sealer |
2 pieces |
N/A |
|

Figure 3: Lentivirus Titer p24 ELISA kit standard curve.

Figure 4 Different concentrations of p24 protein in cell culture media were detected by traditional p24 ELISA kit or Lentivirus Titer p24 ELISA Kit Pro

Figure 5 Samples containing different ratios of free p24 and purified lentivirus were detected by traditional p24 ELISA kit or Lentivirus Titer p24 ELISA Kit Pro

Figure 6 Different lentivirus samples at the same production stage were analyzed by traditional p24 ELISA kit or Lentivirus Titer p24 ELISA Kit Pro



For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.