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目录产品 » 稳定细胞系 » Human Recombinant Endothelin Receptor Type A Stable Cell Line
CHO-K1/EDNRA Stable Cell Line

Figure 1. Endothelin 1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRA cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist endothelin 1. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of endothelin 1 (Mean ± SEM, n = 3). The EC50 of endothelin 1 on this cell was 0.10 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/EDNRA Stable Cell Line

In mammals, the endothelin (ET) family comprises three endogenous isoforms: ET-1, ET-2, and ET-3. These endothelium-derived peptides perform their functions via two endothelin receptors, classified as EDNRA and EDNRB. EDNRA receptors are mainly localized in the vascular smooth muscle cells, where they are the predominant sub-type, and mediate vasoconstriction and proliferation. Selective EDNRA antagonists are effective in the treatment of heart failure, essential hypertension, pulmonary hypertension, and atherosclerosis.
M00149
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human endothelin receptor type A (EDNRA) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/EDNRA Stable Cell Line
    • CHO-K1/EDNRA Stable Cell Line

    Figure 1. Endothelin 1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRA cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist endothelin 1. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of endothelin 1 (Mean ± SEM, n = 3). The EC50 of endothelin 1 on this cell was 0.10 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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