Figure 1: ET-1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB cells. The cells were loaded with Calcium-4 prior to stimulation with an EDNRB receptor agonist ET-1. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ET-1 (Mean ± SD, n = 2). The EC50 of ET-1 on EDNRB expressing CHO-K1 cells was 41.5 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom
CHO-K1/EDNRB Stable Cell Line
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