Figure 1: Bradykinin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/B2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a B2 receptor agonist, bradykinin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of bradykinin (Mean ± SD, n = 2). The EC50 value of bradykinin on this cell was 45.58 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/B2/Gα15 Stable Cell Line
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