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目录产品 » 稳定细胞系 » Human Recombinant NTS1 Neurotensin Receptor Stable Cell Line
CHO-K1/NTS1 Stable Cell Line

Figure 1. Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist neurotensin. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 3). The EC50 of neurotensin on this cell was 7.92 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/NTS1 Stable Cell Line

Neurotensin receptor 1 (NTS1) is a member of G-protein coupled receptor family A and is a receptor for Neurotensin (NT). Neurotensin (NT) exerts its intracellular effect by interacting with 3 different receptors. Two of these receptors (NTR1 and NTR2) belong to the G protein-coupled receptor family, whereas the third one (NTR3) is a type I receptor with a single transmembrane domain. The NTS1 is expressed in CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system, and esophageal smooth muscle.
M00194
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human neurotensin receptor 1 (NTS1) on the surface and contain high levels of G protein Gαq and Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS.
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma).

图例
  • CHO-K1/NTS1 Stable Cell Line
    • CHO-K1/NTS1 Stable Cell Line

    Figure 1. Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist neurotensin. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 3). The EC50 of neurotensin on this cell was 7.92 nM.
    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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