| Product Description |
Recombinant CHO-K1/CRF2/Gα15
(cGMP) cells stably overexpress
human corticotropin releasing factor receptor (CRF2) on the surface and contain
high levels of G protein (Gs) to couple with the
receptor in downstream signaling pathways. |
| Applications |
Functional assay for
CRF2 receptor (cAMP accumulation assay) |
| Expressed Gene |
Codon optimized from NM_001883.5; no expressed tags
|
| Target Protein |
NP_001874.2
|
| Host Cell |
CHO-K1
|
| Size |
Two
vials of frozen cells (1×106 per vial in 1 mL)
|
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received.
|
| Mycoplasma Status |
Negative. The mycoplasma test was performed with MycoAlert™ PLUS
Mycoplasma Detection Kit (Cat.
# LT07-318,
Lonza). |
| Stability |
Stable through more
than 16 passages with no significant changes in assay performance or expression
profile. |
| Culture Properties |
Adherent
|
| Freeze Medium |
45% culture medium,
45% FBS (Cat. # A5669701, Gibco), 10%
DMSO (Cat. #D2650, Sigma) |
| Complete Growth Medium |
Ham’s F-12 K
(Kaighn’s) (Cat. #21127, Life Technologies), 10% FBS |
| Culture Medium |
Ham’s F-12 K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco),
200 μg/ml Hygromycin B (Cat. #10687010, Invitrogen) |
| Gene Synonyms |
CRF-RB; CRHR2; CRFR2; HM-CRF
|
| Background |
The
corticotropin-releasing factor receptor 2 CRF2 is Gs-coupled GPCRs expressed in
the brain, blood vessels and intestine that bind to corticotropin-releasing
factor (CRF). The CRF is a 41-amino acid peptide that plays an important role
in the integration of autonomic, neuroendocrine, and behavioral responses to
stress. GenScript generated a clonal human CRF2-expressing cell line co-expressing
Gα15 in the CHO-K1 host cells. |
Figure 1: Dose dependent stimulation of intracellular cAMP accumulation upon treatment with Urocortin in CHO-K1/CRF2/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. # 62AM4PEC, Cisbio) and intracellular cAMP in CHO-K1/CRF2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by PHERAstar (BMG). The EC50 of Urocortin on CHO-K1/CRF2/Gα15 cells was 0.0865 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is Ratio (665/620) and starts at Bottom and goes to Top along a sigmoid curve.
For research use only. Not intended for human or animal clinical trials,
therapeutic or diagnostic use.
如您需要获取细胞系产品的说明书，请点击“Manual”，完善信息后点击“提交”。
黄金城集团会第一时间联系回复您。注意：中国地区购买的产品，说明书需要以中文站为准。
您也可拨打400-0258686转5810，或发邮件至product@nrapc.com联系黄金城集团。
更多产品文件类型 >>
