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目录产品 » 稳定细胞系 » Human Recombinant Platelet Activating Factor Receptor Stable Cell Line
CHO-K1/PTAFR Stable Cell Line

Figure 1. PAF-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTAFR cells. The cells were loaded with Calcium-4 prior being stimulated with agonist PAF. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PAF (Mean ± SEM, n = 3). The EC50 of PAF on this cell was 0.13 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/PTAFR Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human platelet activating factor receptor (PTAFR) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
M00262
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human platelet activating factor receptor (PTAFR) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/PTAFR Stable Cell Line
    • CHO-K1/PTAFR Stable Cell Line

    Figure 1. PAF-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTAFR cells. The cells were loaded with Calcium-4 prior being stimulated with agonist PAF. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of PAF (Mean ± SEM, n = 3). The EC50 of PAF on this cell was 0.13 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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