Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/H2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a H2 receptor agonist, Histamine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (10-fold dilution) of Histamine (Mean ± SD, n = 2). The EC50 of Histamine on this cell was 89 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
![CHO-K1/H2/Gα15 Stable Cell Line CHO-K1/H2/Gα15 Stable Cell Line](/product/documents/down?doc_name=M00306-cAMP-H2.png&file=scm_files/productFile_notes/2023/03/03/921934e5-cfa6-4640-b292-2c99aca83a50.png)
CHO-K1/H2/Gα15 Stable Cell Line
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