Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EP2/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with EP2 agonist, PGE2. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of PGE2 (mean ± SEM, n = 3). The EC₅₀ of PGE2 on CHO-K1/EP2/Gα15 cells was 15.34 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with PGE2 in CHO-K1/EP2/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/EP2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of PGE2 on CHO-K1/EP2/Gα15 cells was 2.43 nM.

CHO-K1/EP2/Gα15 Stable Cell Line

Prostaglandin (PG) E2 exerts its actions by acting on a group of G-protein-coupled receptors (GPCRs). There are four GPCRs responding to PGE2 designated subtypes EP1, EP2, EP3, and EP4 and multiple splicing isoforms of the subtype EP3. The EP subtypes exhibit differences in signal transduction, tissue localization, and expression regulation. Studies have identified that EP2 mediates many processes, such as facilitating ovulation and fertilization, suppressing dendritic cell differentiation, and promoting amyloid-β formation in Alzheimer's disease.

产品编号	M00311
规格	2 vials 大单询价
数量
价格	询价


联系黄金城集团

产品简介

Product Description	Recombinant CHO-K1 cells stably overexpress human prostaglandin E receptor 2 (PTGER2) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies).
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例

Figure 1. PGE2-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EP2/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with EP2 agonist, PGE2. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of PGE2 (mean ± SEM, n = 3). The EC₅₀ of PGE2 on CHO-K1/EP2/Gα15 cells was 15.34 nM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with PGE2 in CHO-K1/EP2/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/EP2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of PGE2 on CHO-K1/EP2/Gα15 cells was 2.43 nM.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

黄金城集团

CHO-K1/EP2/Gα15 Stable Cell Line

资源中心

技术支持

关于黄金城集团