Figure 1. PTH (1-34)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTH/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PTH (1-34). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of PTH (1-34) (Mean ± SD, n = 2). The EC50 of PTH (1-34) on this cell was 0.26 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
![CHO-K1/PTH1/Gα15 Stable Cell Line CHO-K1/PTH1/Gα15 Stable Cell Line](/product/documents/down?doc_name=M00315-cAMP-PTH1.png&file=scm_files/productFile_notes/2023/03/03/4b867da6-3609-4ee7-8261-a7bb58057548.png)
CHO-K1/PTH1/Gα15 Stable Cell Line
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M00315 |
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