Figure 1. Orexin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OX2 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist orexin A. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of orexin A (Mean ± SD, n = 2). The EC50 of orexin A on this cell was 1.3 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
![CHO-K1/OX2 Stable Cell Line CHO-K1/OX2 Stable Cell Line](/product/documents/down?doc_name=M00316-IP-One-OX2.png&file=scm_files/productFile_notes/2023/03/03/c5dfa753-ab9b-4de0-b8bd-71228e5379ac.png)
CHO-K1/OX2 Stable Cell Line
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