Figure 1. Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D5/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist dopamine. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of dopamine (Mean ± SD, n = 2). The EC50 of dopamine on this cell was 6.5 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/D5/Gα15 Stable Cell Line
Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4). D5 receptor has a 10-fold higher affinity for dopamine than the D1 receptor.
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