黄金城集团

目录产品 » 稳定细胞系 » Human Recombinant Adenosine A2B Receptor Stable Cell Line
CHO-K1/ADORA2B/Gα15 Stable Cell Line

Figure 1. NECA-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADORA2B/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist NECA. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NECA (Mean ± SD, n = 2). The EC50 of NECA on this cell was 0.33 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/ADORA2B/Gα15 Stable Cell Line

CHO-K1/ADORA2B/Gα15 Stable Cell Line

CHO-K1/ADORA2B/Gα15 Stable Cell Line

The adenosine receptors ADORA2B is Gs and Gq/11-coupled GPCR expressed in the Large intestine, cecum, urinary bladder. ADORA2B receptor mediates relaxation to adenosine in human small coronary arteries which is independent of NO but dependents in part on a K+-sensitive mechanism. Pharmacological or molecular biologic activation of ADORA2B receptor may prevent glomerular remodeling associated with glomerulosclerosis, renal disease, and abnormal growth associated with hypertension and diabetes.
M00329
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Synonyms

A2B receptor, A2b, adenosine A2B receptor, A2b, AA2BR, A2BR, A2BAR

Applications Functional assay for ADORA2B receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

  • CHO-K1/ADORA2B/Gα15 Stable Cell Line
  • CHO-K1/ADORA2B/Gα15 Stable Cell Line

    Figure 1. NECA-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADORA2B/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist NECA. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NECA (Mean ± SD, n = 2). The EC50 of NECA on this cell was 0.33 µM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

  • CHO-K1/ADORA2B/Gα15 Stable Cell Line
  • CHO-K1/ADORA2B/Gα15 Stable Cell Line

  • CHO-K1/ADORA2B/Gα15 Stable Cell Line
  • CHO-K1/ADORA2B/Gα15 Stable Cell Line


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