Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC2/Gα15 cells. The cells were loaded with Calcium-4 prior being stimulated with agonist ACTH. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SEM, n = 3). The EC₅₀ of ACTH on this cell was 0.13 μM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with ACTH in CHO-K1/MC2/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/MC2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of ACTH on CHO-K1/MC2/Gα15 cells was 0.36 nM.

CHO-K1/MC2/Gα15 Stable Cell Line

The melanocortin receptor 2, MC2 receptor, is Gs-coupled GPCRs expressed in zona fasciculata of the adrenal cortex placental and stimulates production of cortisol. MC2 is a member of the rhodopsin family of 7-transmembrane and it's also known as the ACTH receptor or corticotropin receptor because it is specific for ACTH alone. Activation of the MC2 receptor initiates a cascade of events affecting multiple steps in corticoid steroidogenesis. Mutations in MC2 may result in familial glucocorticoid deficiency, a group of autosomal recessive disorders characterized by resistance to ACTH.

产品编号	M00336
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant CHO-K1 cells stably overexpress human melanocortin 2 receptor (MC2R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties	Adherent.
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Life Technologies), 100 μg/ml Hygromycin B (Life Technologies)
Complete Growth Medium	Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA	45% Ham’s F-12K (Kaighn’s) (Life Technologies), 45% FBS (Life Technologies), 10% DMSO (Sigma).

图例

Figure 1. ACTH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MC2/Gα15 cells. The cells were loaded with Calcium-4 prior being stimulated with agonist ACTH. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of ACTH (Mean ± SEM, n = 3). The EC₅₀ of ACTH on this cell was 0.13 μM.

Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape.

Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with ACTH in CHO-K1/MC2/Gα15 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/MC2/Gα15 cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC₅₀ of ACTH on CHO-K1/MC2/Gα15 cells was 0.36 nM.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

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CHO-K1/MC2/Gα15 Stable Cell Line

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