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目录产品 » 稳定细胞系 » Human Recombinant 5-HT7 Serotonin Receptor Stable Cell Line
293/5-HT7/CRE-Luc Stable Cell Line

Figure 1. 5-HT-induced luciferase expression in HEK293/5-HTR7/CRE-Luc cells. After stimulation by agonist 5-HT for 6 hours, the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of 5-HT (Mean ± SEM, n = 3). The EC50 of 5-HT on this cell was 16.07 nM.

293/5-HT7/CRE-Luc Stable Cell Line

5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal, and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). 5-HT7 receptor is a Gs-coupled GPCR which is expressed in the heart and brain. Splice variants of the 5-HT7 receptor have been found in the human brain, 5-HT7(a), 5-HT7(b) and 5-HT7(d). Differing only in the lengths of their carboxy terminal, these variants are all functional receptors. GenScript's cloned human 5-HT7-expressing cell line is generated in the 293/CRE-Luc host.
M00423
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产品简介
Product Description Recombinant HEK293 cells stably overexpress human 5-hydroxytryptamine receptor 7 (HTR7) on the surface and contain high levels of G protein Gαs to couple with the inducible luciferase in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium DMEM, 10% FBS
Freeze Medium-DATA 50% FBS (Cat. No. 10099-141, Life Technologies), 45% DMEM (Cat. No. 10566, Life Technologies) 5% DMSO (Cat. No. D2650, Sigma)

图例
  • 293/5-HT7/CRE-Luc Stable Cell Line
    • 293/5-HT7/CRE-Luc Stable Cell Line

    Figure 1. 5-HT-induced luciferase expression in HEK293/5-HTR7/CRE-Luc cells. After stimulation by agonist 5-HT for 6 hours, the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of 5-HT (Mean ± SEM, n = 3). The EC50 of 5-HT on this cell was 16.07 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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