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目录产品 » 稳定细胞系 » Human Recombinant Bile Acid Receptor 1 Stable Cell Line
CHO-K1/GPBAR1 Stable Cell Line

Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with lithocholic acid (LCA) in CHO-K1/GPBAR1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/GPBAR1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of LCA on CHO-K1/GPBAR1 cells was 3.34 μM.

CHO-K1/GPBAR1 Stable Cell Line

The gene GPBRA1 encodes G protein-coupled bile acid receptor. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production.
M00432
询价

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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human G protein-coupled bile acid receptor 1 (GPBAR1) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/GPBAR1 Stable Cell Line
    • CHO-K1/GPBAR1 Stable Cell Line

    Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with lithocholic acid (LCA) in CHO-K1/GPBAR1 cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEB; Revvity) and intracellular cAMP in CHO-K1/GPBAR1 cells competitively bind with Europium Cryptate-labeled anti-cAMP antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of LCA on CHO-K1/GPBAR1 cells was 3.34 μM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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