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CHO-K1/ADRA2B/Gqi5 Stable Cell Line

Figure 1. UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2B/Gqi5 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304 (Cat. No. U104-5MG; Sigma). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of UK14304 (Mean ± SEM, n = 3). The EC50 of UK14304 on this cell was 0.12 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/ADRA2B/Gqi5 Stable Cell Line

Adrenoceptors are 7-transmembrane receptors which mediate the central and peripheral actions of the neurotransmitter, noradrenaline (norepinephrine), and the hormone and neurotransmitter, adrenaline (epinephrine). Based on both pharmacological and molecular evidence, they are divided into three major types - α1, α2, and β. The α2 adrenoceptors include 3 subtypes - α2A, α2B, and α2C - each of which couples to Gi/o protein primarily. The α2B adrenoceptors (ADRA2B) is expressed in the kidney, liver, brain, lung, heart, and skeletal muscle. The development of α2B and α2C knock-out mice has shown that these two subtypes are not involved in the central hypotensive response to α2 agonists.
M00454
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human adrenoceptor alpha 2B (ADRA2B) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stability: Stable through more than 15 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/ADRA2B/Gqi5 Stable Cell Line
    • CHO-K1/ADRA2B/Gqi5 Stable Cell Line

    Figure 1. UK14304-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/ADRA2B/Gqi5 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist UK14304 (Cat. No. U104-5MG; Sigma). The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of UK14304 (Mean ± SEM, n = 3). The EC50 of UK14304 on this cell was 0.12 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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