黄金城集团

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CHO-K1/Lag3 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human LAG3 on CHO-K1/LAG3 cells. The CHO-K1/LAG3 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD223 antibody (Cat. No. 12-2239, eBioscience).

CHO-K1/Lag3 Stable Cell Line

Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene.[1] LAG3, which was discovered in 1990[2] and was designated CD223 (cluster of differentiation 223) after the Seventh Human Leucocyte Differentiation Antigen Workshop in 2000[3]. LAG-3 is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.
M00532
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human lymphocyte activating 3 (LAG3) on the surface. The surface expression of LAG3 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against LAG3 or to measure binding affinity between LAG3 and anti-LAG3 antibodies.
Culture Properties Adherent.
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/Lag3 Stable Cell Line
    • CHO-K1/Lag3 Stable Cell Line

    Figure 1. FACS analysis of cell surface expression of human LAG3 on CHO-K1/LAG3 cells. The CHO-K1/LAG3 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD223 antibody (Cat. No. 12-2239, eBioscience).


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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