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Figure 1: FACS analysis of cell surface expression of VISTA on CHO-K1/VISTA cells. The CHO-K1/VISTA cells (red) and the negative control CHO-K1 cells (green and blue) were probed using PE-human VISTA (Cat. No. FAB71261P, R&D).

CHO-K1/VISTA Stable Cell Line

Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.

产品编号	M00533
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description	Recombinant CHO-K1 cells stably overexpress V-domain Ig suppressor of T cell activation (VISTA) on the cell surface. The surface expression of VISTA is validated by FACS analysis. This cell line is designed for cell-based binding for screening antibodies binding with VISTA and evaluating target binding affinity.
Applications	Cell-based binding assay or use as immunogen

产品简介

Expressed Gene	Codon optimized from NM_022153.1; no expressed tags
Target Protein	NP_071436
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza).
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	95% Complete growth medium, 5% DMSO (Cat. No.D2650, Sigma)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

技术背景

Gene Synonyms

B7H5, GI24, B7-H5, Dies1, PD-1H, SISP1, VISTA, PP2135, C10orf54, DD1alpha

Background

V-domain Ig suppressor of T cell activation (VISTA) is a potent negative regulator of T-cell function that is expressed on hematopoietic cells. VISTA levels are heightened within the tumor microenvironment, in which its blockade can enhance antitumor immune responses in mice. In humans, blockade of the related programmed cell death 1 (PD-1) pathway has shown great potential in clinical immunotherapy trials. Here, we report the structure of human VISTA and examine its function in lymphocyte negative regulation in cancer.

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