目录产品 » 稳定细胞系 » Human Recombinant CD80 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of human CD80 on CHO-K1/CD80 cells. The CHO-K1/CD80 cells (blue) and the negative control CHO-K1 cells (red) were probed using mouse anti-human CD80 antibody (17F3) (Cat. No. A01973, Genscript) and FITC-conjugated goat anti-mouse IgG (Cat. No. ab6785, Abcam).

CHO-K1/CD80 Stable Cell Line

Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells.

产品编号	M00545
规格	2 vials 大单询价
数量
价格	询价


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产品简介

Product Description	Recombinant CHO-K1 cells stably overexpress human CD80 on the surface. The surface expression of CD80 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against CD80 or to measure binding affinity between CD80 and anti-CD80 antibodies.
Culture Properties	Adherent.
Stability	Stability: Stable through more than 16 passages without significant changes in assay performance or expression profile.
Size	Two vials of frozen cells (>1×10⁶ per vial in 1 mL).
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件

Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies).
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies).
Freeze Medium-DATA	95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).