黄金城集团

目录产品 » 稳定细胞系 » Human Recombinant VPAC2 Receptor Stable Cell Line
CHO-K1/CRE-Luc/VPAC2 Stable Cell Line

Figure 1. VIP-induced luciferase expression in CHO-K1/CRE-Luc/VPAC2 cells. After stimulation by VIP for 6 hours, the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of VIP (Mean ± SEM, n = 3). The EC50 of VIP on cells was 0.50 nM.

CHO-K1/CRE-Luc/VPAC2 Stable Cell Line

The Vasoactive intestinal polypeptide receptor 2 (VPAC2) is a Gs-coupled receptor expressed in the stroma of uterus and prostate; smooth muscles in gastrointestinal tract, seminal vesicles and skin; blood vessels; thymus. VPAC2 is an essential regulator of the circadian pacemaker of the hypothalamic suprachiasmatic nuclei.
M00547
询价

联系黄金城集团
产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human vasoactive intestinal peptide receptor 2 (VPAC2) and luciferase gene which can be translated under the control of cAMP response element (CRE). This product contains high levels of G protein Gαs to couple with the VPAC2 receptor in downstream signaling pathway (cAMP/PKA).
Culture Properties Adherent.
Stability Stability: Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies), 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS.
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma).

图例
  • CHO-K1/CRE-Luc/VPAC2 Stable Cell Line
    • CHO-K1/CRE-Luc/VPAC2 Stable Cell Line

    Figure 1. VIP-induced luciferase expression in CHO-K1/CRE-Luc/VPAC2 cells. After stimulation by VIP for 6 hours, the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of VIP (Mean ± SEM, n = 3). The EC50 of VIP on cells was 0.50 nM.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


喜欢新升级的网站吗？

讨厌

不喜欢

一般

喜欢

非常喜欢

*
Skip